JCHOR
Journal of Current Hematology & Oncology Research

The Journal of Current Hematology & Oncology Research regularly publishes internationally qualified research in hematology and oncology within the current scholarly knowledge.

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Original Article
The relationship between serum vitamin b12 level and fibroadenoma in adolescent female patients: a retrospective case-control study
Abstract
Aims: This study aimed to investigate serum vitamin B12 levels in adolescent female patients diagnosed with fibroadenoma and to evaluate whether a significant difference exists between fibroadenoma patients and healthy controls.
Methods: This retrospective case-control study was conducted between 2018 and 2023. A total of 46 adolescent female patients diagnosed with fibroadenoma based on ultrasonographic evaluation in accordance with ACR guidelines, with histopathological confirmation in eight cases (17%), and 46 age- and sex-matched controls were included. Hematological parameters were analyzed alongside serum vitamin B12 levels. Univariate and multivariate logistic regression analyses were performed to identify independent predictors of fibroadenoma. A decision tree model was additionally constructed to define clinically meaningful threshold values.
Results: The mean serum vitamin B12 level was significantly lower in the fibroadenoma group compared to controls (304.54 ± 79 pg/mL vs. 407 ± 137 pg/mL; p<0.001). Among fibroadenoma patients, 88.9% had vitamin B12 levels below 300 pg/mL. The proportion of patients with vitamin B12 levels exceeding 340 pg/mL was 18.9% in the fibroadenoma group versus 81.1% in controls, a statistically significant difference (p = 0.019). Multivariate logistic regression identified low serum vitamin B12 level as an independent predictor of fibroadenoma. Decision tree analysis confirmed vitamin B12 as the primary discriminating variable, with red cell distribution width (RDW) emerging as a secondary node.
Conclusion: To our knowledge, this is the first study to investigate serum vitamin B12 levels in adolescent patients with fibroadenoma. The significantly lower vitamin B12 levels observed in fibroadenoma patients compared to controls suggest a potential role of vitamin B12 in the pathogenesis of this benign breast lesion. The identified 300 pg/mL threshold suggests that serum vitamin B12 may serve as a simple and accessible biomarker for fibroadenoma risk stratification in adolescents. Prospective multicenter studies incorporating functional B12 markers such as homocysteine and methylmalonic acid are needed to validate these findings.

Keywords : Fibroadenoma, vitamin B12, adolescent, breast diseases, biomarkers

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Ethical Statement

<p>This study was approved by the Scientific Researches Evaluation and Ethics Committee of Ankara Etlik City Hospital (Date: 27.11.2024, Decision No: AEŞH BADEK-2024-1132).</p>

Informed Consent

<p>This retrospective study used pre-existing anonymized patient data. No additional intervention was performed, and there was no direct patient contact. The study was approved by the Ethics Committee, and the requirement for written informed consent was waived by the ethics committee.</p>

Author Contributions

<p>Concept: GŞ; Design: MBH, GŞ; Control: GŞ, ŞY; Resources: ŞY, AF; Materials: MBH, CK; Data Collection and/or Processing: MBH; Analysis and/or Interpretation: MBH, GŞ; Literature Review: MBH; Writing the Article: MBH; Critical Review: MBH, ŞY, SE, CK, AF, GŞ.</p>

Conflict of Interest

<p>The authors declare no conflicts of interest related to this study.</p>

Financial Disclosure

<p>The authors received no financial support for the conduct or publication of this research.</p>


1. Lee M, Soltanian HT. Breast fibroadenomas in adolescents: current perspectives. Adolesc Health Med Ther. 2015;6:159-163. doi:10.2147/AHMT.S55833
2. Dupont WD, Page DL, Parl FF, et al. Long-term risk of breast cancer in women with fibroadenoma. N Engl J Med. 1994;331(1):10-15. doi:10.1056/NEJM199407073310103
3. El-Wakeel H, Umpleby HC. Systematic review of fibroadenoma as a risk factor for breast cancer. Breast. 2003;12(5):302-307. doi:10.1016/S0960-9776(03)00123-1
4. Lyon P, Strippoli V, Fang B, Cimmino L. B vitamins and one-carbon metabolism: implications in human health and disease. Nutrients. 2020;12(9):2867. doi:10.3390/nu12092867
5. Plazar N, Jurdana M. Hyperhomocysteinemia and the role of B vitamins in cancer. Radiol Oncol. 2010;44(2):79-85. doi:10.2478/v10019-010-0022-z
6. Wu K, Helzlsouer KJ, Comstock GW, Hoffman SC, Nadeau MR, Selhub J. A prospective study on folate, B12, and pyridoxal 5’-phosphate (B6) and breast cancer. Cancer Epidemiol Biomarkers Prev. 1999;8(3):209-217.
7. Choi SW. Vitamin B12 deficiency: a new risk factor for breast cancer? Nutr Rev. 1999;57(8):250-253. doi:10.1111/j.1753-4887.1999.tb06952.x
8. Lajous M, Lazcano-Ponce E, Hernandez-Avila M, Willett W, Romieu I. Folate, vitamin B6, and vitamin B12 intake and the risk of breast cancer among Mexican women. Cancer Epidemiol Biomarkers Prev. 2006;15(3): 443-448. doi:10.1158/1055-9965.EPI-05-0532
9. Houghton SC, Eliassen AH, Zhang SM, et al. Plasma B-vitamin and one-carbon metabolites and risk of breast cancer before and after folic acid fortification in the United States. Int J Cancer. 2019;144(8):1929-1940. doi:10.1002/ijc.31934
10. Zeng J, Gu Y, Fu H, Liu C, Zou Y, Chang H. Association between one-carbon metabolism-related vitamins and risk of breast cancer: a systematic review and meta-analysis of prospective studies. Clin Breast Cancer. 2020;20(4):e469-e480. doi:10.1016/j.clbc.2020.02.012
11. Klein KA, Kocher M, Lourenco AP, et al. ACR Appropriateness Criteria palpable breast masses: 2022 update. J Am Coll Radiol. 2023;20(5S): S146-S163. doi:10.1016/j.jacr.2023.02.013
12. Essén A, Santaolalla A, Garmo H, et al. Baseline serum folate, vitamin B12 and the risk of prostate and breast cancer using data from the Swedish AMORIS cohort. Cancer Causes Control. 2019;30(6):603-615. doi:10.1007/s10552-019-01170-6
13. Haghighat G, Khajeh-Mehrizi A, Ranjbar H. Evaluation of serum vitamin B12 levels in patients with colon and breast cancer: a case-control study. Int J Hematol Oncol Stem Cell Res. 2023;17(4):240-244. doi:10.18502/ijhoscr.v17i4.13914
14. Vashi P, Edwin P, Popiel B, Lammersfeld C, Gupta D. Methylmalonic acid and homocysteine as indicators of vitamin B-12 deficiency in cancer. PLoS One. 2016;11(1):e0147843. doi:10.1371/journal.pone.0147843
Article available in :
Volume 4, Issue 4, 2026
Page : 140-145
https://doi.org/10.51271/JCHOR-0098
Article Information
Received : 09 Jul 2026
Accepted : 19 Aug 2026
Published : 13 Nov 2026
Corresponding Author :

Melda Berber Hamamcı: Department of Pediatric Oncology, Faculty of Medicine, Ankara University, Ankara, Turkiye

[email protected]
Citation : Berber Hamamcı M, Yeşil Ş, Ergişi S, Kılcı C, Fettah A, Gürses Ş. The relationship between serum vitamin B12 level and fibroadenoma in adolescent female patients: a retrospective case-control study. J Curr Hematol Oncol Res. 2026;4(4):140-145. doi:10.51271/JCHOR-0098

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